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M9480862.TXT
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1994-09-05
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Document 0862
DOCN M9480862
TI p24 Antigen is not primarily virion-associated in peripheral blood of
HIV-infected individuals: a comparison with quantitation of HIV RNA.
DT 9410
AU Wilber J; Yeghiazarian T; Todd J; Urdea M; Chiron Corporation,
Emeryville, CA.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:483 (abstract no. T-8). Unique
Identifier : AIDSLINE ASM94/94313083
AB The level of p24 antigen has been used as a surrogate marker for the
level of viremia in HIV-infected patients. To understand the extent to
which p24 antigen (p24Ag) is associated with virions, EDTA plasma was
collected from 12 HIV-infected individuals and assayed for the quantity
of virion-associated HIV p24Ag and RNA. Since there are numerous
published observations that free RNA is highly unstable in plasma, it
was assumed that detectable HIV RNA was contained in virions. Samples
were tested for HIV RNA using branched DNA signal amplification
(Quantiplex HIV-RNA, Chiron Corporation, Emeryville, California) and for
p24Ag using the immune complex-dissociation (ICD) modification of the
p24Ag Assay (Coulter Corporation, Hialeah, Florida). Virions were
separated from two 1 ml aliquots of each plasma sample by centrifugation
at 23,000 g at 2-8 C for 1 hr. RNA and p24 Ag were measured in whole
plasma and supernatant, and RNA was measured in reconstituted pellets.
As shown in Figure 1, a mean of 85% of p24Ag remained in the supernatant
after virions were pelleted. The ratio of virion-associated to free
p24Ag varied from patient to patient. These results may help explain
previously reported inconsistencies between infectious virus titer and
p24Ag quantitation. The source of non-virion-associated p24Ag could be
degraded virus or whole virus-independent production of antigen.
Variation in p24Ag levels could be due to strain-dependent differences
in these processes and not to changes in virus load, which may lead to
problems in interpretation. TABULAR DATA, SEE ABSTRACT VOLUME.
DE Gene Amplification Human HIV/*ISOLATION & PURIF/PHYSIOLOGY HIV
Antigens/BLOOD HIV Core Protein p24/*BLOOD HIV Infections/*BLOOD RNA,
Viral/ANALYSIS/GENETICS Virion/*ISOLATION & PURIF/PHYSIOLOGY MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).